PCR Supplies
ATCC Acholeplasma laidlawii
Acholeplasma laidlawii strain PG8 [NCTC 10116, PG8; A] is a whole-genome sequenced bacterial type strain isolated from sewage. This strain is recommended as a test microorganism for USP <63>.
Midwest Scientific PR1MA SmartGlow DNA Safe Stain, 1mL1 Pre-stain, Blue and UV Light
PR1MA SmartStain PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA SmartStain PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
Roche Diagnostics KAPA2G Fast PCR Kits - HotStart PCR Kit, with dNTPs, 500 U
KAPA2G Fast PCR Kits - HotStart PCR Kit, with dNTPs, 500 U.
New England Biolabs, Inc. LunaScript RT SuperMix - 100 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 g and as low as single copies of RNA.
INTACT GENOMICS INC Taq DNA Polymerase 5000 Units include dNTP
Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Applications Routing PCR cloning Primer extension Colony PCR Elongation efficiency 1.0-1.2 kb/min. Formulated for amplifying long target DNA. Efficient for amplifying high GC content DNA with Intact Genomics magic enhancer
INTACT GENOMICS INC T4 DNA Polymerase 500 Units
IG™ T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. Applications 3´-overhang removal to form blunt ends (1, 2). 5´-overhang fill-in to form blunt ends (1, 2). Probe labeling using replacement synthesis (2). DNA library preparation for Next-generation sequencing. Ligation-independent cloning of PCR products. Second strand synthesis in site-directed mutagenesis (3).
New England Biolabs, Inc. Bst-XT WarmStart™ DNA Polymerase (Glycerol-free) - 8000 units
Bst-XT WarmStart™ DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows. Bst-XT WarmStart DNA Polymerase is an engineered variant of Bacillus stearothermophilus DNA Polymerase, Large Fragment fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. This enzyme combines the polymerization speed of Bst 3.0 and the high specificity of Bst 2.0 DNA polymerases. Bst-XT WarmStart DNA Polymerase contains a reversibly bound aptamer, which inhibits polymerase activity at room temperature. The aptamer rapidly releases above 45°C, therefore no separate activation step is needed. Bst-XT WarmStart DNA Polymerase contains 5' to 3' DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5' to 3' and 3' to 5' exonuclease activity.
Agilent Technologies PASSIVE REFERENCE DYE
Passive Reference Dye, QPCR Reagents, Reagent for any fluorescent chemistry, Wide linear dynamic range of amplification, Passive reference dye included in a separate tube to maximize performance on different instrument platforms
Alkali Scientific Radiant™ Probe 1-Step No-ROX qPCR Kit, 100 Reactions, (1 X 1mL 2X Mix, 1 X 0.2mL 20X RTase)
Alkali Scientific offers Radiant™ Probe 1-Step No-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step No-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
New England Biolabs, Inc. LunaScript RT SuperMix - 2500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 g and as low as single copies of RNA.
OriGene GAPDH Human qPCR Primer Pair (NM_002046), 200 reactions
qSTAR qPCR primer pairs against Homo sapiens gene GAPDH, 200 reactionsLocus ID: 2597UniProt ID: P04406Synonyms:G3PD; GAPD; HEL-S-162ePComponents: 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.Quality Control: The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.Storage: Store at -20°C.Stable: The primer mix is stable for one year from date of shipping.